Product Description
Composition of kit:
HLTS1001-1: 5 ml × 10 specially made human lymphocyte separating tubes, 10 ml lymphocyte separating solution: PBS contains 2% FBS 10 ml; Lymphocyte cryopreservation solution 5ml, 10 cell cryopreservation tubes
This product is a sterilized aqueous solution with opalescence or microemulsion. The main components are dextran and meglumine diatrizoate. It is applicable to the separation of required cells from blood and tissue homogenate, and is widely used in medical treatment and medical biology.
Other human and animal cell isolates with various specific gravity. Note: Because the cell dispersion coefficient and cell charge of the separating solution with different species and different specific gravity are different, the user should provide the specific gravity of the required separating solution, the species of animals and the name of the separated cell.
matters needing attention:
1) The product should be stored at 4 ° C after opening.
2) After the cell isolation solution is taken out of the refrigerator, it can not be used immediately. When the solution temperature rises to room temperature, it needs to be mixed well before use.) During cell separation, control the temperature at 18-28 ° C, otherwise the separation effect will be affected.
Application:
Isolation of Lymphocytes from Human Peripheral Blood and Cord Blood
Dominance:
Pour out directly after centrifugation, without absorbing, simple and convenient:
Prevent PBMC loss and high recovery rate
Survival rate of isolated PBMC>95%
Characteristic:
Density change rate shall be in accordance with Ficll400, pantothenic acid and sodium hydroxide.
The optimum separation solution density is 1.077g/ml (Ng/ml)
Physiological parameters
High density at low viscosity
Sterile, ready to use, solution
Product specification:
Solution: separating liquid
PH:7.0-7.5
Osmotic pressure: 280-340mOsmol/kg
Endotoxin:<5EU/ml
Sterility: tested
Shelf life: 2 years
Storage: 12C - 20 ° C
Be careful:
The cell separator of our company is light sensitive, and it should be treated in a dark way during transportation and storage.
Storage:
Store at 12 - 20 ° C away from light, and store at 4 ° C after unsealing.
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